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Surface antigen and polypseudopodia in abortive transformation of BHK 21 cells by polyoma virus

✍ Scribed by M. G. P. Stoker; Maureen Thornton; P. Riddle; Françoise Birg; G. Meyer

Publisher
John Wiley and Sons
Year
1972
Tongue
French
Weight
479 KB
Volume
10
Category
Article
ISSN
0020-7136

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✦ Synopsis

Abstract

When BHK 21 cells are exposed to high multiplicities of polyoma virus, a change in surface antigen may be detected by immunofluorescence, beginning at 18 h and affecting up to 88% of cells 24 h after infection. Time‐lapse cinematography shows that there is a concurrent change in movement and shape (polypseudopodia) of the cells in the confluent layer, involving about the same proportion of cells. Both these changes appear before the virus‐induced stimulation of the thymidine incorporation and mitosis, and before the increase in lectin agglutinability. The S antigen disappears more rapidly than the polyspeudopodia in these abortively transformed cells, but both persist in stably transformed cells. The antigenic change and polyspeudopodia are not the result of the virus‐induced stimulation of cell DNA synthesis and mitosis, but it is not yet possible to say if they are an essential requirement for this stimulation.

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