Optically pure amino-acids were labelled with tritium by the Wilzbach method. The percentage of radioactivity found in the opposite enantiomer was determined by reverse dilution analysis. After tritiation of L-phenylalanine, D-phenylalanine, L-tyrosine, L-proline, D-proline, L-glutamic acid and L-al
Application of tritium high resolution NMR spectroscopy to analysis of tritium-labelled amino acids and peptides
โ Scribed by S. G. Rosenberg; Yu. A. Zolotarev; N. F. Myasoedov
- Book ID
- 104949640
- Publisher
- Springer
- Year
- 1992
- Tongue
- English
- Weight
- 422 KB
- Volume
- 3
- Category
- Article
- ISSN
- 0939-4451
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๐ SIMILAR VOLUMES
Optically pure L-valine was labelled with tritium by the Wilzbach method. Only 4 of the activity of the radiochemically pure valine was found in D-valine. This figure was determined by reverse isotope dilution. The suitability of this method for valine was tested, wich good results, with DL-valine
A small-scale synthesis of high specific activity, N-(2-chloro-2-[3H]-ethyl)-N'-cyclohexyl-N-nitrosourea ([3H]-CCNU) has been accomplished from tritium-labelled ethanolamine. The product is pure by TLC and HPLC analysis and has been used successfully to modify DNA. The overall yield on radioactivity