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Tritium labelling of amino-acids and peptides by the Wilzbach method. II. Racemization of amino-acids during exposure to tritium gas

✍ Scribed by J. H. Parmentier

Publisher: John Wiley and Sons
Year: 1966
Tongue: French
Weight: 377 KB
Volume: 2
Category: Article
ISSN: 0022-2135
DOI: 10.1002/jlcr.2590020405

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✦ Synopsis

Optically pure amino-acids were labelled with tritium by the Wilzbach method. The percentage of radioactivity found in the opposite enantiomer was determined by reverse dilution analysis. After tritiation of L-phenylalanine, D-phenylalanine, L-tyrosine, L-proline, D-proline, L-glutamic acid and L-alanine respectively 2.7, 2.2, < 1.1, 4.6, 4.0, 11.9 and 19.3 of the acticities of the radiochemically pure amino-acids were found in D-phenylalanine, L-phenylalanine, D-tyrosine, D-proline, L-proline, D-glutamic acid and D-alanine.

Wilzbach labelling of phenylalanine caused the formation of a considerable amount of a radiochemical impurity that was identijied as P-cyclohexylalanine.

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