Resolution of tritium-labelled amino acid racemates by ligand-exchange chromatography. Part 1. Method for obtaining L- and D-[3H]valine using a polystyrene resin with L-hydroxyproline groupings
✍ Scribed by Myasoedov N. F.; Kuznetsova O. B.; Petrenik O. V.; Davankov V. A.; Zolotarev Yu. A.
- Publisher
- John Wiley and Sons
- Year
- 1980
- Tongue
- French
- Weight
- 384 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0022-2135
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✦ Synopsis
Abstract
Ligand‐exchange chromatography is shown to be applicable for obtaining the optical isomers of [^3^H]valine. Data are presented showing the effect of the asymmetric sorbent's structure on its kinetic properties and the optimum conditions selected for the chromatographic resolution of D, L‐[^3^H] valine. The method makes it possible to obtain both isomers of [^3^H]valine with an optical purity close to 100% in about 2 hours with a simultaneous radiochemical purification. The method does not require any preliminary chemical modification of the amino acid and does not impose any limitations to specific activity values.