## Abstract Intrinsic primordial germ cells (PGCs) from stage 27 (5βdayβold) chick embryonic germinal ridges were cultured in vitro for a further 5 days, and shown to proliferate on stroma cells derived from the germinal ridge. To determine whether these cultured PGCs could colonize and contribute
Appearance of Primordial Germ Cells in Young Chick Blastoderms Cultured in vitro : (primordial germ cells/blastoderms/chick)
β Scribed by L. A. SUTASURYA; S. YASUGI; T. MIZUNO
- Book ID
- 108942528
- Publisher
- John Wiley and Sons
- Year
- 1983
- Tongue
- English
- Weight
- 345 KB
- Volume
- 25
- Category
- Article
- ISSN
- 0012-1592
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Fluorescent reagentβlabelled PGCs isolated from the blood of 2βdayβold chick embryos were cultured on stroma cells derived from 5βdayβold germinal ridge in Medium 199 supplemented with 10% FBS, human IGFβ1, bovine FGFβb, and murine LIF. In 7 experiments, the number of MCs increased by an average of
## Abstract Primordial germ cells (PGCs) of the early chick embryo were examined using scanning electron microscopy. Temporal changes in the form and distribution of surface projections were found to be correlated with migratory phases of PGCs. Nonβmigrating PGCs were spherical to ovoid with relati