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Proliferation of chick primordial germ cells cultured on stroma cells from the germinal ridge.

✍ Scribed by Il-Kuk Chang; Atsushi Tajima; Tateki Chikamune; Tadao Ohno

Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
471 KB
Volume
19
Category
Article
ISSN
1065-6995

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✦ Synopsis

Fluorescent reagent‐labelled PGCs isolated from the blood of 2‐day‐old chick embryos were cultured on stroma cells derived from 5‐day‐old germinal ridge in Medium 199 supplemented with 10% FBS, human IGF‐1, bovine FGF‐b, and murine LIF. In 7 experiments, the number of MCs increased by an average of 4.8 fold in 4 days. Intrinsic PGCs in the 5‐day embryonic germinal ridge were observed loosely attached to the stroma cells, and they also increased 3.8 fold during culture for 4 days. These results indicate the possibility of applying this culture method to the production of transgenic chickens.

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