Attraction of chick primordial germ cells by gonadal anlage in vitro
โ Scribed by Kuwana, Takashi ;Maeda-Suga, Hitomi ;Fujimoto, Toyoaki
- Publisher
- John Wiley and Sons
- Year
- 1986
- Tongue
- English
- Weight
- 434 KB
- Volume
- 215
- Category
- Article
- ISSN
- 0003-276X
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Gonadal primordial germ cells (gPGCs) were collected from gonadal anlage of 5day-old White Leghorn (WL) embryos. Collected gPGCs were suspended in freezing medium containing 10% dimethyl sulphoxide (DMSO). The cell-suspension was frozen at 1ยฐC/min until the temperature reached -80ยฐC; cells were then
Experiments were conducted to elucidate the factor that influences the concentration of circulating primordial germ cells (cPGCs) in two-day old chick embryos. The concentration of cPGCs was observed to be highest at stage 14 (66.9 ยฑ 23.2 ยตl) and decreased thereafter. However, considerable egg to eg
Adhesive interactions with the surrounding cells and extracellular matrix components are crucial for migration and development of primordial germ cells. The possibility of isolating and culturing such cells from the mouse embryo has allowed the initial characterization of their adhesion properties,