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Isolation, characteristics, and long-term culturing of chicken gonadal primordial germ cells and blastodermal cells

โœ Scribed by N. M. Suraeva; E. A. Vorotelyak; M. I. Prokofiev; A. V. Samoilov; A. V. Vasiliev; V. V. Terskikh; A. Yu. Baryshnikov


Book ID
110159511
Publisher
Springer
Year
2008
Tongue
English
Weight
265 KB
Volume
423
Category
Article
ISSN
0012-4966

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Gonadal primordial germ cells (gPGCs) were collected from gonadal anlage of 5day-old White Leghorn (WL) embryos. Collected gPGCs were suspended in freezing medium containing 10% dimethyl sulphoxide (DMSO). The cell-suspension was frozen at 1ยฐC/min until the temperature reached -80ยฐC; cells were then