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SIMPLE SEPARATION OF CHICKEN GONADAL PRIMORDIAL GERM CELLS WITH AND WITHOUT FOREIGN GENES

โœ Scribed by Dong Kee Jeong; Yeong Ho Hong; Jae Yong Han


Book ID
102966490
Publisher
Elsevier Science
Year
2002
Tongue
English
Weight
513 KB
Volume
26
Category
Article
ISSN
1065-6995

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Gonadal primordial germ cells (gPGCs) were collected from gonadal anlage of 5day-old White Leghorn (WL) embryos. Collected gPGCs were suspended in freezing medium containing 10% dimethyl sulphoxide (DMSO). The cell-suspension was frozen at 1ยฐC/min until the temperature reached -80ยฐC; cells were then