Anti–double-stranded DNA antibodies, nucleosomes, and systemic lupus erythematosus: A time for new paradigms?
✍ Scribed by O. P. Rekvig; J. C. Nossent
- Publisher
- John Wiley and Sons
- Year
- 2003
- Tongue
- English
- Weight
- 196 KB
- Volume
- 48
- Category
- Article
- ISSN
- 0004-3591
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We describe a patient with systemic lupus erythematosus who, after a disease flare, sequentially produced antibodies to specific Sm polypeptides (demonstrated by counterimmunoelectrophoresis, immunoblotting, and RNA immunoprecipitation) concomitantly with the disappearance of anti-double-stranded DN
An anti-double-stranded (ds) DNA antibodies in serum-detection system was developed based on flow immunoassay with packed-capillary column. Alkaline phosphatase conjugated DNA (ALP-DNA) and anti-dsDNA antibody were separated from unreacted ALP-DNA on the basis of the difference in isoelectric point.