An improved method for determination of total hydroxyproline in urine
โ Scribed by A.C. Parekh; D.H. Jung
- Book ID
- 115765990
- Publisher
- Elsevier Science
- Year
- 1970
- Tongue
- English
- Weight
- 602 KB
- Volume
- 4
- Category
- Article
- ISSN
- 0006-2944
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
The determination of hydroxyproline has become increasingly important in view of its use in the detection of metabolic bone disease and the measurement of collagen turnover under a variety of experimental conditions. The most commonly used method is the one described by Prockop and Udenfriend (1). I
An HPLC method for measuring total hydroxyproline in human urine was validated. Hydroxyproline derivatization was achieved with 9-fluorenylmethyl chloroformate after blocking primary amino acids with orthophthaldialdehyde. The derivatives (hydroxyproline and internal standard) were separated by reve