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Automated determination of total hydroxyproline in urine: Use of internal standard

โœ Scribed by James D. Jones; Allan C. Skalet; Philip C. Burnett

Publisher
Elsevier Science
Year
1970
Tongue
English
Weight
221 KB
Volume
37
Category
Article
ISSN
0003-2697

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An automated measurement of total and free hydroxyproline in serum or urine is presented that uses flow injection analysis. After exclusion of nonspecific substances, hydroxyproline was oxidized by chloramine-T and L-cysteine with Ehrlich's reagent. The linearity obtained was from 3.8kmole/ L to 1.