Measurement of free and total hydroxyproline by automated flow injection of serum or urine samples from maintenance hemodialysis patients with renal osteodystrophy
✍ Scribed by Dr. Yoshinori Uji; Arthur Karmen; Hiroaki Okabe; Keishi Hata; Masakazu Miura; Kazuyuki Ozaki; Mitsuo Minamizaki; Tetsushi Shibata; Seiichi Inayama
- Publisher
- John Wiley and Sons
- Year
- 1994
- Tongue
- English
- Weight
- 369 KB
- Volume
- 8
- Category
- Article
- ISSN
- 0887-8013
No coin nor oath required. For personal study only.
✦ Synopsis
An automated measurement of total and free hydroxyproline in serum or urine is presented that uses flow injection analysis. After exclusion of nonspecific substances, hydroxyproline was oxidized by chloramine-T and L-cysteine with Ehrlich's reagent.
The linearity obtained was from 3.8kmole/ L to 1.22 mmole/L with good precision (CV <3%). Comparison of the proposed method with HPLC yielded ~0 . 9 3 9 as the correlation coefficient.
Reference intervals of free and total hydroxyproline are 1.4-9.7 kmole/L, 3.8-27.2 pmole/L for serum, and 10.0-72.5 p,mole/L, 25.2-303.6 ,umole/L for urine, respectively. Serum free and total hydroxyproline levels in renal osteodystrophy patients on maintenance hemodialysis (N=71) were significantly higher than in controls (P<O.OOOl). This method is superior to the use of HPLC with regard to stability of the color reaction. The measurement of serum free and total hydroxyproline is a useful marker for therapeutic observation of renal osteodystrophy patients.