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A simple and automated HPLC method for determination of total hydroxyproline in urine. Comparison with excretion of pyridinolines

✍ Scribed by S. Castelain; S. Kamel; C. Picard; G. Desmet; J.L. Sebert; M. Brazier

Book ID
115828160
Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
472 KB
Volume
235
Category
Article
ISSN
0009-8981

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A simple and automated HPLC method for d
A simple and automated HPLC method for determination of total hydroxyproline in urine. Comparison with excretion of pyridinolines
✍ S. Castelain; S. Kamel; C. Picard; G. Desmet; J.L. Sebert; M. Brazier πŸ“‚ Article πŸ“… 1995 πŸ› Elsevier Science 🌐 English βš– 472 KB

An HPLC method for measuring total hydroxyproline in human urine was validated. Hydroxyproline derivatization was achieved with 9-fluorenylmethyl chloroformate after blocking primary amino acids with orthophthaldialdehyde. The derivatives (hydroxyproline and internal standard) were separated by reve