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An enzymatic assay for the detection of natural cytotoxicity

โœ Scribed by K.J. McCormick; B.C. Giovanella; D.C. Day; W.R. Parsons; J.S. Stehlin


Book ID
118897989
Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
403 KB
Volume
35
Category
Article
ISSN
0022-1759

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An assay for the enzymatic synthesis of
โœ M.E. Levitch ๐Ÿ“‚ Article ๐Ÿ“… 1977 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 238 KB

A method is described for the determination of the enzyme-catalyzed synthesis of y-glutamylmethylamide (GMAD). The assay involves conversion of '"C-labeled methylamine to ["'C] CMAD, addition of cold alcohol to stop the reaction, and plating an aliquot of the reaction mixture on planchets with alkal