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An enzymatic assay of plasma phenylalanine and tyrosine for the detection and management of phenylketonuria

✍ Scribed by Rong-sen Shen; C.Joan Richardson; Bobbye M. Rouse; Creed W. Abell


Book ID
115766955
Publisher
Elsevier Science
Year
1981
Tongue
English
Weight
583 KB
Volume
26
Category
Article
ISSN
0006-2944

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L-[13N]Tyrosine and L-[13N]phenylalanine were synthesized using immobilized enzymes by two methods. In method 1, [13N]ammonia is converted to L-[13N]glutamate; transamination with p-hydroxyphenylpyruvate yields L-[13N]tyrosine. [13N]Tyrosine is separated from other labeled intermediates on a Poropak