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Methods for the enzymatic synthesis of tyrosine and phenylalanine labeled with nitrogen-13

✍ Scribed by Alan S. Gelbard; Arthur J.L. Cooper; Yasuhisa Asano; Edward Nieves; Sabina Filc-Dericco; Karen C. Rosenspire

Publisher: Elsevier Science
Year: 1990
Weight: 535 KB
Volume: 41
Category: Article
ISSN: 0883-2889
DOI: 10.1016/0883-2889(90)90114-v

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✦ Synopsis

L-[13N]Tyrosine and L-[13N]phenylalanine were synthesized using immobilized enzymes by two methods. In method 1, [13N]ammonia is converted to L-[13N]glutamate; transamination with p-hydroxyphenylpyruvate yields L-[13N]tyrosine. [13N]Tyrosine is separated from other labeled intermediates on a Poropak Q column. In method 2, phenylalanine dehydrogenase catalyzes the reversible reductive [13N]amination of either phenylpyruvate or p-hydroxyphenylpyruvate to form L-[13N]phenylalanine or L-[13N]tyrosine, respectively. The feasibility of labeling DOPA and tryptophan with 13N was also demonstrated.

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