An assay for picomole levels of tyrosine and related phenols and its application to the measurement of phenylalanine hydroxylase activity
β Scribed by Steven W. Bailey; June E. Ayling
- Publisher
- Elsevier Science
- Year
- 1980
- Tongue
- English
- Weight
- 666 KB
- Volume
- 107
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
Attack of hydroxyl radical (.OH), generated by a Fenton system at physiological pH, upon L-phenylalanine produces three isomeric tyrosines, o-tyrosine (2-hydroxyphenylalanine), m-tyrosine (3-hydroxyphenylalanine), and p-tyrosine (4-hydroxyphenylalanine). These may be separated by high-performance li
## Abstract A new type of fluorescence assay for the determination of peroxidase (POx) activity is presented. The assay is based on the indication of the enzymatic consumption of H~2~O~2~ (HP), using a fluorescent europiumβtetracycline (Eu~3~TC) complex as indicator. On addition of HP, this complex