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Amplified luminometric assays of alkaline phosphatase using riboflavin phosphates

✍ Scribed by Harborn, Stuart ;Eggelte, Henny J. ;Benson, Suzanne M. ;Rabin, Brain R.

Publisher
John Wiley and Sons
Year
1991
Weight
488 KB
Volume
6
Category
Article
ISSN
0884-3996

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✦ Synopsis

An asasy for alkaline phosphatase is described which is based on the hydrolysis of riboflavin phosphates (5'FMN or 4 F M N ) t o produce riboflavin. This is converted t o 5'FMN using riboflavin kinase. and then asayed using the bacterial bioluminescent system from Vibrio harveyi or V. fischeri. The most sensitive assay is obtained using 4 F M N . which can measure less than 20amol after a I-hour incubation.

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