Lucigenin is popular as a chemiluminescent reagent. It produces strong chemiluminescence by reaction with hydrogen peroxide or some organic reductants. The application of this intense chemiluminescence has been studied for high-sensitive enzymatic activity assay. Phenacyl phosphate was synthesized a
โฆ LIBER โฆ
Chemiluminescent assay of alkaline phosphatase using dihydroxyacetone phosphate as substrate detected with lucigenin
โ Scribed by Amane Kokado; Hidetoshi Arakawa; Masako Maeda
- Publisher
- John Wiley and Sons
- Year
- 2002
- Tongue
- English
- Weight
- 122 KB
- Volume
- 17
- Category
- Article
- ISSN
- 1522-7235
- DOI
- 10.1002/bio.669
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The chemiluminescent reaction of lucigenin w i t h various reducing sugars and reducing compounds has been studied. It was found that dihydroxyacetone gave the most intense chemiluminescence (CL). We have developed highly sensitive chemiluminescent methods for alkaline phosphatase (ALP) based on the