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Chemiluminescent assay of alkaline phosphatase using phenacyl phosphate

✍ Scribed by Hidehiko Sasamoto; Masako Maeda; Akio Tsuji

Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
456 KB
Volume
306
Category
Article
ISSN
0003-2670

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✦ Synopsis

Lucigenin is popular as a chemiluminescent reagent. It produces strong chemiluminescence by reaction with hydrogen peroxide or some organic reductants. The application of this intense chemiluminescence has been studied for high-sensitive enzymatic activity assay. Phenacyl phosphate was synthesized as a new substrate for alkaline phosphatase (ALP); the phenacyl alcohol formed in the enzyme-catalyzed reaction reacted to lucigenin giving luminescence. The detection limit for ALP was 3.4 X IO-"' mol per assay tube after overnight incubation. ALP is used for enzyme immunoassays as a labeling enzyme. The assay in the present study should be applicable to high-sensitivity enzyme immunoassays.

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