✦ LIBER ✦

An Amplified Chemiluminescent Assay for the Detection of Alkaline Phosphatase

✍ Scribed by M. Fisher; S. Harbron; B.R. Rabin

Publisher: Elsevier Science
Year: 1995
Tongue: English
Weight: 474 KB
Volume: 227
Category: Article
ISSN: 0003-2697
DOI: 10.1006/abio.1995.1254

No coin nor oath required. For personal study only.

✦ Synopsis

An amplification assay for the measurement of alkaline phosphatase has been combined with a luminescent end point using the luminol-peroxidase system to produce the first enzyme-amplified chemiluminescent assay based on the principle of prosthetogenesis. This assay is both quantitative and extremely sensitive. When the assay was used to detect alkaline phosphatase in solution, the detection limit was 0.4 amol in a (5-\mathrm{min}) assay. The interassay variance ranged from 4 to (20 %) and 7 to (19 %) across the dynamic range of the assay for a chemiluminescent assay and an enhanced chemiluminescent assay, respectively, employing two different preparations of luminol. 厄 1995 Academic Press, Inc.

📜 SIMILAR VOLUMES

Chemiluminescent determination of leukoc

Chemiluminescent determination of leukocyte alkaline phosphatase: an advantageous alternative to the cytochemical assay

✍ Marília P.P. Kanegae; Valdecir F. Ximenes; Roberto P. Falcão; Virgílio A.R. Colt 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 134 KB

## Abstract The determination of leukocyte alkaline phosphatase (LAP) is used as an aid to diagnose many diseases in the laboratory. For example, it can be used to distinguish chronic myeloid leukemia (CML) from other myeloproliferative disorders (particularly myelofibrosis and polycythemia) and le

Chemiluminescent assay of alkaline phosp

Chemiluminescent assay of alkaline phosphatase using dihydroxyacetone phosphate as substrate detected with lucigenin

✍ Amane Kokado; Hidetoshi Arakawa; Masako Maeda 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 122 KB

An optimized method for the chemilumines

An optimized method for the chemiluminescent detection of alkaline phosphatase levels during osteodifferentiation by bone morphogenetic protein 2

✍ Jeremy S. Blum; Rebecca H. Li; Antonios G. Mikos; Michael A. Barry 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 144 KB 👁 2 views

G-rich sequence-functionalized polystyre

G-rich sequence-functionalized polystyrene microsphere-based instantaneous derivatization for the chemiluminescent amplified detection of DNA

✍ Liang Xin; Zhijuan Cao; Choiwan Lau; Masaaki Kai; Jianzhong Lu 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 542 KB

## Abstract Herein, we develop a novel chemiluminescence (CL) approach with high sensitivity and excellent selectivity, by taking advantage of magnetic beads as preconcentration carriers and polystyrene microspheres as an amplification platform. Briefly, a ‘sandwich‐type’ detection strategy is empl

Using an enzyme linked immunosorbent ass

Using an enzyme linked immunosorbent assay (ELISA) and a protein phosphatase inhibition assay (PPIA) for the detection of microcystins and nodularins

✍ Wayne W. Carmichael; Jisi An 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 96 KB

Development of an ELISA method for detec

Development of an ELISA method for detecting immune complexes between tissue-nonspecific alkaline phosphatase and immunoglobulin G

✍ Kazuo Hocchi; Tatsuya Ohashi; Toshihide Miura; Kumiko Sasagawa; Yasuhito Sato; F 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 267 KB

## Abstract A convenient method for measuring immune complexes between tissue‐nonspecific alkaline phosphatase (TNSALP) and immunoglobulin G (IgG) (i.e., TNSALP‐IgG) would be highly useful for routine analyses. Here, we identified a surface‐active agent that would dissolve membrane but not dissocia