Potentiometric determination of inorganic phosphate using a salicylate-sensitive membrane electrode and an alkaline phosphatase enzyme

✍ Scribed by Takashi Katsu; Takashi Kayamoto

• Publisher: Elsevier Science
• Year: 1992
• Tongue: English
• Weight: 275 KB
• Volume: 265
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(92)85147-x

✦ Synopsis

A method for determmmg morgamc phosphate usmg a sabcylate-sensltlve membrane electrode 1s described,, based on the detectlon of sahcylate formed by enzymatic hydrolysis of o-carboxyphenyl phosphate by alkahne phosphatase m the presence of phosphate The phosphate InhIbIted competltlvely the formation of sabcylate to an extent Inversely proportlonal to the concentration of added phosphate, detected down to 0 05 mM The method was successfully applied to the determmatlon of phosphate m blood serum