Amperometric Enzyme Electrode for the Determination of Aspartame in Diet Food

✍ Scribed by R.L. Villarta; A.A. Suleiman; G.G. Guilbault

• Publisher: Elsevier Science
• Year: 1993
• Tongue: English
• Weight: 247 KB
• Volume: 48
• Category: Article
• ISSN: 0026-265X
• DOI: 10.1006/mchj.1993.1071

✦ Synopsis

An amperometric enzyme electrode for the determination of aspartame in protein-free samples has been constructed. It is based on the coimmobilization of an aspartamehydrolyzing enzyme, aspartate aminotransferase, and glutamate oxidase on an Immobilon membrane. The electrode response is linear from (2.0 \times 10^{-4} M) to (1.5 \times 10^{-3} M) with a current slope of (2.1 \mathrm{nA} / \mathrm{m} M). The electrode was used to successfully measure aspartame in some dietary products. 1993 Academic Press, Inc.