𝔖 Bobbio Scriptorium
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Enzyme electrode for the determination of bilirubin

✍ Scribed by Alain Fortuney; George G. Guilbault

Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
374 KB
Volume
8
Category
Article
ISSN
1040-0397

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✦ Synopsis

Bilirubin oxidase (EC 1.3.3.5) catalyzes the oxidation of bilirubin to biliverdin with the concurrent reduction of oxygen to hydrogen peroxide. A platinum electrode was prepared with an immobilized bilirubin oxidase membrane. Bilirubin was determined by monitoring the increase in hydrogen peroxide concentration from the enzyme reaction. The calibration plot for bilirubin shows linearity up to 300 pM. The detection limit is 0.7 pM.

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