An enzyme electrode for on-line determination of ethanol and methanol

✍ Scribed by Hafedh Belghith; Jean-Louis Romette; Daniel Thomas

• Publisher: John Wiley and Sons
• Year: 1987
• Tongue: English
• Weight: 356 KB
• Volume: 30
• Category: Article
• ISSN: 0006-3592
• DOI: 10.1002/bit.260300902

✦ Synopsis

Since a stable alcohol oxidase with a high specific activity is not commercially available, we propose to produce and purify this enzyme from a strain of the yeast Han- senula polymorpha. This alcohol oxidase was immobilized into a gelatin matrix and its activity was estimated by a pOz sensor. The enzyme electrode obtained was then used in a continuous flow system to measure methanol or ethanol concentrations. The sample oxygen content dependence of the signal was minimized by the support properties. Measuring time for each sample were less than two minutes including response data treatment and rinsing step. The enzyme electrode re- sponse was set for ethanol from 0.5mM to 15mM and for methanol from 10mM to 300mM. On repeated use, the electrode signal for 10mM of ethanol was stable for at least 500 assays. Analysis have been performed in different beverages such as wine and beer, and the results compared to those obtained with classical methods of analysis.