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Amino acid requirements for attachment and outgrowth of the mouse blastocyst in vitro

✍ Scribed by R. B. L. Gwatkin

Publisher: John Wiley and Sons
Year: 1966
Tongue: English
Weight: 673 KB
Volume: 68
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1040680313

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✦ Synopsis

Arginine, cystine, histidine, leucine and threonine were needed for outgrowth of the mouse blastocyst in vitro. Omission of lysine, methionine, phenylalanine, tryptophane and tyrosine from the culture medium markedly reduced blastocyst outgrowth, h u t did not inhibit it completely; while omission of isoleucine and valine reduced the extent of outgrowth only slightly. Blastocysts kept for seven days i n a free-floating condition by omitting arginine and leucine from the medium, grew out when these amino acids were added. Such behavior may be analogous to delayed implantation in utero and suggests that the free amino acid content of the u t c p s could be a n important factor i n the control of implantation. Blastocysts delayed from implanting i n the uterus by ovariectomy were activated to outgrowth in a complete medium, but the intracellular changes associated with outgrowth occurred more slowly than i n undelayed blastocysts.

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