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Effect of extracellular K+ on hatching and outgrowth of mouse blastocysts in vitro.

✍ Scribed by Zhengzhu Jin; Meishan Jin; Godfried M. Roomans

Publisher: Elsevier Science
Year: 1994
Tongue: English
Weight: 205 KB
Volume: 18
Category: Article
ISSN: 1065-6995
DOI: 10.1006/cbir.1994.1127

No coin nor oath required. For personal study only.

✦ Synopsis

ABSTRACT

The dependence of blastocyst development on the extracellular Na^+^/K^+^ ratio was investigated in an in vitro system. Hatching and outgrowth of mouse blastocysts was enhanced at Na^+^/K^+^ ratios between 3 and 10 compared to the ratio of about 25 typical for most culture media and serum. At a Na^+^/K^+^ ratio of 2, blastocyst hatching and outgrowth were inhibited. The requirement of blastocyst development for relatively high extracellular K^+^ concentrations agrees with the fact that K^+^ concentrations in oviduct and uterine secretions are higher than in serum. The findings can also be relevant in optimizing in vitro culturing techniques for blastocysts.

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