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Energy requirement for the incorporation of amino acids into protein of isolated mouse-liver mitochondria

✍ Scribed by Patrick F. Delaney; Norman J. Girardot

Publisher
John Wiley and Sons
Year
1966
Tongue
English
Weight
238 KB
Volume
68
Category
Article
ISSN
0021-9541

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✦ Synopsis

Mitochondria isolated from mouse liver can incorporate amino acids into mitochondrial protein. Studies with oligomycin and antihistamine drugs indicate that this incorporation may not be an ATP requiring process.

Several laboratories '55; Truman and Korner, '62; Bronk, '63; Kalf, '63) have reported that isolated mitochondria incorporate amino acids into mitochondrial protein without the addition of supernatant fractions. Truman and Korner ('62) and Bronk ('63) have demonstrated that oxidizable substrate provided a better energy source for this process than added adenosine triphosphate (ATP).

Bronk ('63) found that 0.3 vg/ml of oligomycin did not inhibit mitochondrial incorporation of amino acid into protein, even though this concentration of oligomycin blocked the terminal phosphorylation steps of ATP formation. Bronk also

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