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Synthesis of Alaninyl and N-(2-Aminoethyl)glycinyl Amino Acid Derivatives Containing the Green Fluorescent Protein Chromophore in Their Side Chains for Incorporation into Peptides and Peptide Nucleic Acids

✍ Scribed by Thorsten Stafforst; Ulf Diederichsen

Publisher: John Wiley and Sons
Year: 2007
Tongue: English
Weight: 294 KB
Volume: 2007
Category: Article
ISSN: 1434-193X
DOI: 10.1002/ejoc.200600729

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✦ Synopsis

Abstract

Artificial amino acids carrying either the chromophore of the Green Fluorescent Protein (GFP) or a modification as their side chains have been synthesized: Boc‐protected alaninyl derivatives and Fmoc‐protected N‐(2‐aminoethyl)glycine‐functionalized amino acids were obtained and could be applied in solid‐phase peptide synthesis. The incorporation of the GFP chromophore into N‐(2‐aminoethyl)glycine‐PNA was achieved and fluorescence was studied as a function of hybridization with complementary DNA. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2007)

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Cover Picture: Synthesis of Alaninyl and

Cover Picture: Synthesis of Alaninyl and N-(2-Aminoethyl)glycinyl Amino Acid Derivatives Containing the Green Fluorescent Protein Chromophore in Their Side Chains for Incorporation into Peptides and Peptide Nucleic Acids (Eur. J. Org. Chem. 6/2007)

✍ Thorsten Stafforst; Ulf Diederichsen 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 134 KB 👁 2 views

## Abstract **The cover picture shows** a chromophore of the Green Fluorescent Protein, which undergoes fast internal conversion by rotation causing the quenching of its fluorescence in solution. In contrast, the intact protein shows a high fluorescence quantum yield and there is still a need to un