✦ LIBER ✦

Amino acid requirements for growth of the rabbit blastocyst in vitro

✍ Scribed by Joseph C. Daniel Jr.; R. Sivarama Krishnan

Publisher: John Wiley and Sons
Year: 1967
Tongue: English
Weight: 453 KB
Volume: 70
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1040700204

No coin nor oath required. For personal study only.

✦ Synopsis

Ten amino acids, namely, arginine, histidine, lysine, tryptophane, methionine, phenylalanine, leucine, valine, threonine and serine were indispensable for growth of rabbit blastocysts i n vitro; others were nonessential. Of all the essential amino acids, arginine and lysine were required in relatively high concentrations, 10-2 M and 10-3 M, respectively, for optimum growth. Complete omission of the nonessential amino acids from the medium markedly reduced blastocyst growth. Interaction between serine and glycine demonstrated a partial sparing action on serine by glycine, similar to that observed between methionine and cysteine. The amino acid composition of a culture medium capable of providing continuous and consistent growth of rabbit blastocysts i n vitro is described.

📜 SIMILAR VOLUMES

The effects of water-soluble vitamins on

The effects of water-soluble vitamins on the expansion of rabbit blastocysts in vitro

✍ Kane, Michael T. 📂 Article 📅 1988 🏛 John Wiley and Sons 🌐 English ⚖ 321 KB 👁 1 views

The vitamin requirements for culture of rabbit morulae to expanded blastocysts were examined. Early morulae were cultured for 5 days either in a control complete medium containing all the 11 water-soluble vitamins of F10 culture medium (biotin, pantothenate, choline, inositol, niacinamide, pyridoxin

In vitro uptake and incorporation of ami

In vitro uptake and incorporation of amino acids by blastocysts from intact and ovariectomized mice

✍ Weitlauf, Harry M. 📂 Article 📅 1973 🏛 John Wiley and Sons 🌐 English ⚖ 449 KB 👁 1 views

## Abstract Blastocysts were recovered from the uteri of intact or ovariectomized mice and incubated with a mixture of ^14^C amino acids in vitro. The level of uptake and incorporation of the amino acids as estimated by scintillation counting was higher in blastocysts from intact than in those from

The effects of some amino acids on growt

The effects of some amino acids on growth and lipid production in Inonotus obliquus grown in vitro

✍ K. Kahlos 📂 Article 📅 1994 🏛 John Wiley and Sons 🌐 English ⚖ 750 KB

## Abstract The effect of the distribution of free and esterified fatty acids, including triterpenes and sterols, on the mycelial growth of the basidiomycete __Inonotus obliquus__ (Pers. ex Fr.) Pilat in malt extract and solid supplemented mineral media was investigated. The amino acids __DL__‐alan

In vitro study of the metabolic effects

In vitro study of the metabolic effects of D-amino acids

✍ Nuran Ercal; Xiang Luo; Richard H. Matthews; Daniel W. Armstrong 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 537 KB

While the L-configuration of amino acids predominates in all known living systems, D-enantiomers of amino acids have been detected with highly sensitive chromatographic techniques in human physiological fluids. In the present study, the survival of Chinese hamster ovary cells (CHO) and HeLa cells wa

Energy requirement for the incorporation

Energy requirement for the incorporation of amino acids into protein of isolated mouse-liver mitochondria

✍ Patrick F. Delaney; Norman J. Girardot 📂 Article 📅 1966 🏛 John Wiley and Sons 🌐 English ⚖ 238 KB 👁 1 views

Mitochondria isolated from mouse liver can incorporate amino acids into mitochondrial protein. Studies with oligomycin and antihistamine drugs indicate that this incorporation may not be an ATP requiring process. Several laboratories '55; Truman and Korner, '62; Bronk, '63; Kalf, '63) have reported

Evidence for a broadly-specific, amino a

Evidence for a broadly-specific, amino acid requiring dehydrogenase at the pyruvate branchpoint in sea anemones

✍ Ellington, W. Ross 📂 Article 📅 1979 🏛 John Wiley and Sons 🌐 English ⚖ 353 KB

## Abstract The tissues of a number of species of sea anemones contain an enzyme(s) capable of oxidizing NADH in the presence of pyruvate and either L‐arginine or L‐alanine. The activities using either amino acid coelute on Sephadex G‐100 and DEAE‐Sephadex −50 columns. In addition, the peak DEAE‐Se