Accelerated chromatographic method for determination of hydroxyproline
β Scribed by Paul X. Callahan; Judith A. Shepard; Stanley Ellis
- Book ID
- 107711324
- Publisher
- Elsevier Science
- Year
- 1972
- Tongue
- English
- Weight
- 557 KB
- Volume
- 49
- Category
- Article
- ISSN
- 0003-2697
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π SIMILAR VOLUMES
A continuous automatic method involving alkaline hydrolysis, neutralization with sulfuric acid, and specific detection of 4-hydroxyproline is described. The main parameters of the method are studied. This technique is capable of detecting 10 pmoliliter of Chydroxyproline linked in peptidic sequence
A modification of the method of hydroxyproline determination in proteins was devised. The modification consists of the hydrolysis of proteins in 72% perchloric acid of 100Β°C for 2 hr instead of 20 hr, autoclaving in 6 N HCl or 2 N Ba(OH)\*. Determination of hydroxyproline by the modified method does