Enzymic method for determination of hydroxyproline
โ Scribed by Carmen L. Rosano
- Book ID
- 107710707
- Publisher
- Elsevier Science
- Year
- 1966
- Tongue
- English
- Weight
- 264 KB
- Volume
- 15
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
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An automated procedure has been described based on the manual method of Hagen and Hagen [(1%2) Canad. J. Biochem. 40, 11291 which will rapidly and reproducibly measure glycerol concentrations. The method was developed primarily for the determination of glycerol in adipose tissue and various incubat
1969 (17). \* Abbreviations : DTNB (Ellman's reagent), 5,5'-dithiobis(2-nitrobenzoic acid) ; GSH and GSSG. reduced and oxidized glutathione; NAD(H) and NADP(H), nicotinamide-adenine dinucleotide, nicotinamide-adenine dinucleotide phosphate and their rrduced forms: Tris. tris(hydroxymethyl)aminometha