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An automated enzymic method for the determination of glycerol

โœ Scribed by Michael G. Gore

Publisher
Elsevier Science
Year
1976
Tongue
English
Weight
376 KB
Volume
75
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

An automated procedure has been described based on the manual method of Hagen and Hagen [(1%2) Canad. J. Biochem.

40, 11291 which will rapidly and reproducibly measure glycerol concentrations. The method was developed primarily for the determination of glycerol in adipose tissue and various incubation media. The glycerol in solution is estimated by its partial conversion to dihydroxyacetone by glycerol dehydrogenase from Aerobacter aerogenes.

The range of glycerol concentrations able to be measured is more extensive than with other automated methods. 604

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โœ Endre A. Balazs; Kjell O. Berntsen; Judith Karossa; David A. Swann ๐Ÿ“‚ Article ๐Ÿ“… 1965 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 313 KB

The first practical method for the determination of hexosamines was worked out by Elson and Morgan (1). Since that time many modifications have been introduced in an attempt to increase the sensitivity and reproducibility of the technique (2) and to decrease the effect of interfering substances (3)