An automated method for the determination of hexosamines

✍ Scribed by Endre A. Balazs; Kjell O. Berntsen; Judith Karossa; David A. Swann

• Publisher: Elsevier Science
• Year: 1965
• Tongue: English
• Weight: 313 KB
• Volume: 12
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(65)90222-8

✦ Synopsis

The first practical method for the determination of hexosamines was worked out by Elson and Morgan (1). Since that time many modifications have been introduced in an attempt to increase the sensitivity and reproducibility of the technique (2) and to decrease the effect of interfering substances

(3). The manual procedure, however, even with the numerous modificat.ions, still gives variable results in the hands of different operators, due, probably, to slight variations in the conditions used in the acetylation step of the reaction. In view of the fact that this procedure is widely used in many laboratories in the study of glycoproteins and glycosaminoglycans, it was decided to automate the procedure.3