Improved method of hexosamine determination
β Scribed by A.R. Johnson
- Publisher
- Elsevier Science
- Year
- 1971
- Tongue
- English
- Weight
- 446 KB
- Volume
- 44
- Category
- Article
- ISSN
- 0003-2697
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π SIMILAR VOLUMES
The first practical method for the determination of hexosamines was worked out by Elson and Morgan (1). Since that time many modifications have been introduced in an attempt to increase the sensitivity and reproducibility of the technique (2) and to decrease the effect of interfering substances (3)
Gaseous H,O, is sampled on a Ti(IV)-H,SO, coated filter, where titanium peroxy complex is formed. After sampling the filter is leached in a phosphate buffer (pH 7) and the peroxide concentration analysed spectrophotometrically at 505 nm after having added 4-(2-pyridylazohesorcinol (PAR). The triple