๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

A spectrophotometric assay for dehydroascorbate reductase

โœ Scribed by Robert L. Stahl; Leonard F. Liebes; Charles M. Farber; Robert Silber

Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
307 KB
Volume
131
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

A simple spectrophotometric assay for dehydroascorbate reductase based on the change in absorbance associated with the formation of ascorbic acid is described. Using a partially purified preparation from spinach leaves, the reaction was found to be linear with time and enzyme concentration. The reaction rate determined by this assay correlated well with that obtained by a high-performance liquid chromatography method. Possible advantages over currently available assays as well as potential applications are discussed.

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