A spectrophotometric assay for phosphoribosylpyrophosphate synthetase
โ Scribed by M. Ferrari; A. Giacomello; C. Salerno; E. Messina
- Publisher
- Elsevier Science
- Year
- 1978
- Tongue
- English
- Weight
- 240 KB
- Volume
- 89
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
โฆ Synopsis
A simple, rapid, and sensitive spectrophotometric assay of phosphoribosylpyrophosphate synthetase activity is described. This assay is based on the quantitative measurement of the reaction product AMP by a NADH-coupled enzyme method.
๐ SIMILAR VOLUMES
A simple, continuous assay for aminoacyl-tRNA synthetases utilizing a commercially available pyrophosphate assay reagent kit was demonstrated. The method coupled aminoacyl-tRNA synthetase activity with pyrophosphate-dependent fructose-6-phosphate kinase, aldolase, triosephosphate isomerase, and glyc
A new continuous spectrophotometric assay is demonstrated for Escherichia coli alanyl-tRNA synthetase. It involves beta-gamma adenylyl imidophosphate as a substitute for ATP in the pyrophosphate exchange reaction. The net conversion of beta-gamma adenylyl imidophosphate to ATP can be linked to NADP
A nonradioactive multiwell spectrophotometric assay for the interferon-induced enzyme 2',5'-oligoadenylate synthetase measuring the inorganic pyrophosphate produced during oligoadenylate synthesis has been developed. A coupled enzymatic reaction results in a mole to mole formation of NADPH compared
An orotate phosphoribosyltransferase, OPRTase, assay method which relies upon binding reactant ['Hlorotic acid and product ['H]orotidine-S-monophosphate to polyethyleneimineimpregnated-cellulose resin and collecting on a GFC glass fiber filter is presented. Elution with 2 X 5 ml of 0.1 M sodium chlo