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Continuous spectrophotometric assay for aminoacyl-tRNA synthetases

✍ Scribed by Gu-gang Chang; Foo Pan; Yee-hong Lin; Hsien-yu Wang

Publisher: Elsevier Science
Year: 1984
Tongue: English
Weight: 283 KB
Volume: 142
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(84)90478-0

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✦ Synopsis

A simple, continuous assay for aminoacyl-tRNA synthetases utilizing a commercially available pyrophosphate assay reagent kit was demonstrated. The method coupled aminoacyl-tRNA synthetase activity with pyrophosphate-dependent fructose-6-phosphate kinase, aldolase, triosephosphate isomerase, and glycerophosphate dehydrogenase. PPi formation was correlated with the oxidation of NADH, and was monitored continuously by the decrease of absorbance at 340 nm.

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