A continuous spectrophotometric assay is described for the aminoacylation reaction catalyzed by Escherichia coli alanyl-transfer RNA synthetase. The assay is based on coupling the alanyl-tRNA synthetase-dependent formation of AMP to the lactate dehydrogenase oxidation of NADH. Oxidation of NADH, as
โฆ LIBER โฆ
A semiautomated assay procedure for the determination of aminoacyl-tRNA synthetase activity
โ Scribed by Sylvia A. McCune; P.L. Yu; Walter E. Nance
- Publisher
- Elsevier Science
- Year
- 1977
- Tongue
- English
- Weight
- 301 KB
- Volume
- 79
- Category
- Article
- ISSN
- 0003-2697
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โฆ Synopsis
A semiautomated assay procedure for the determination of aminoacyl-tFWA synthetase activity is described. The assay system employs a Technicon AutoAnalyzer with a continuous filter paper attachment. A larger number of samples can be measured with greater accuracy, reproducibility, and more rapidly than the manual filter paper disk assay system.
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