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Colorimetric assay for aminoacyl-tRNA synthetases

✍ Scribed by Gu-Gang Chang; Foo Pan; Cheng Yeh; Ter-Mei Huang

Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
484 KB
Volume
130
Category
Article
ISSN
0003-2697

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✦ Synopsis

A simple, rapid, and inexpensive procedure for the determination of aminoacyl-tRNA synthetase activity is described. The assay is based on a green color formed between PPi and ammonium molybdate in the presence of mercaptoethanol. The sensitivity is in the nanomole range and is comparable with the conventional [32P]PPi-ATP exchange assay. Amino acid, ATP, magnesium ion, and most common reagents do not interfere with the color yield.

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