A continuous spectrophotometric assay for argininosuccinate synthetase based on pyrophosphate formation
โ Scribed by William E. O'Brien
- Publisher
- Elsevier Science
- Year
- 1976
- Tongue
- English
- Weight
- 437 KB
- Volume
- 76
- Category
- Article
- ISSN
- 0003-2697
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A new continuous spectrophotometric assay is demonstrated for Escherichia coli alanyl-tRNA synthetase. It involves beta-gamma adenylyl imidophosphate as a substitute for ATP in the pyrophosphate exchange reaction. The net conversion of beta-gamma adenylyl imidophosphate to ATP can be linked to NADP
A continuous spectrophotometric assay is described for the aminoacylation reaction catalyzed by Escherichia coli alanyl-transfer RNA synthetase. The assay is based on coupling the alanyl-tRNA synthetase-dependent formation of AMP to the lactate dehydrogenase oxidation of NADH. Oxidation of NADH, as