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A spectrophotometric assay for demethiolating activity

โœ Scribed by Simo Laakso; Veikko Nurmikko

Publisher
Elsevier Science
Year
1976
Tongue
English
Weight
322 KB
Volume
72
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

A rapid method for detection of demethiolating activity in bacterial suspensions is described. The procedure is based on spectrophotometric determination of aryl mercaptan produced by quantitative reaction of 5,5'-dithiobis(2nitrobenzoic acid) (DTNB) with liberating methanethiol. Under the conditions used (3WC, pH 7.4) the reduction of DTNB by methanethiol was linear over 20 min. A linear correlation between demethiolation rate and cell concentration was observed up to 0.5 mg dry wt/ml. DTbJB had no effect on methionine transport or normal growth of Pseudomonas j?uorescens UK-1.

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