๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

A spectrophotometric assay for adenine phosphoribosyltransferase

โœ Scribed by C. Salerno; A. Giacomello

Publisher
Elsevier Science
Year
1977
Tongue
English
Weight
318 KB
Volume
82
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

โœฆ Synopsis

The present paper describes a new spectrophotometric assay for adenine phosphoribosyltransferase activity which is highly reproducible, rapid, sensitive, and simpler than the isotopic assays for this enzyme. This assay is based on the quantitative measurement of the product AMP by a NADH-coupled enzyme method.

๐Ÿ“œ SIMILAR VOLUMES

A spectrophotometric assay for cellobias
A spectrophotometric assay for cellobiase
โœ D.L. Liu; C.C. Walden ๐Ÿ“‚ Article ๐Ÿ“… 1969 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 390 KB

Chemical methods for measuring cellobiase activity are based on increased reducing capacity, following conversion of cellobiose into glucose (l-3). The chemical methods are time consuming, of low sensitivity, and nonspecific. Enzymic methods use glucose oxidase to estimate the amount of glucose libe

A method for assaying orotate phosphorib
A method for assaying orotate phosphoribosyltransferase and measuring phosphoribosylpyrophosphate
โœ D.J. Hupe; N.D. Behrens ๐Ÿ“‚ Article ๐Ÿ“… 1987 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 485 KB

An orotate phosphoribosyltransferase, OPRTase, assay method which relies upon binding reactant ['Hlorotic acid and product ['H]orotidine-S-monophosphate to polyethyleneimineimpregnated-cellulose resin and collecting on a GFC glass fiber filter is presented. Elution with 2 X 5 ml of 0.1 M sodium chlo