We have simplified the highly sensitive silver stain of R. C. Switzer III, C. R. Merril, and S. Shifrin (1979, Anal. Biochem. 98, 231β237) for visualizing proteins in polyacrylamide gels. We have reduced the number of steps in the procedure from 10 to 6, simplified the reagents in each step, and red
A simplified silver diammine method for the staining of nucleic acids in polyacrylamide gels
β Scribed by Dr. Frank Vari; Kevin Bell
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 574 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0173-0835
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## Abstract A sensitive silver staining method using eriochrome blackβ T as a silverβion sensitizer for DNA detection in polyacrylamide gels was developed. The sensitivity of this staining method was significantly improved by the new silverβion sensitizer containing a diazo group, which has reducing
## Methods In this paper, we report a fast, simple, and reproduc-Agarose gel electrophoresis of DNA. Samples of Hinible staining protocol for nucleic acids in agarose gels dIII digests of l DNA and/or plasmidic DNA were run with a sensitivity in the order of 10 pg/mm 2 . It took on a horizontal 0.