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A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels

✍ Scribed by Berl R. Oakley; Donald R. Kirsch; N.Ronald Morris

Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
581 KB
Volume
105
Category
Article
ISSN
0003-2697

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✦ Synopsis

We have simplified the highly sensitive silver stain of R. C. Switzer III, C. R. Merril, and S. Shifrin (1979, Anal. Biochem.
98, 231–237) for visualizing proteins in polyacrylamide gels. We have reduced the number of steps in the procedure from 10 to 6, simplified the reagents in each step, and reduced the amount of silver required by a factor of 10, thus greatly reducing the expense of the procedure. In common with the original silver stain, our procedure is 100 times more sensitive than Coomassie brilliant blue and is comparable in sensitivity to radioautography of radioactively labeled proteins.

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Experimentally improved reliability of ultrasensitive silver staining of protein in polyacrylamide gels
✍ Margret Eschenbruch; Robert R. BΓΌrk πŸ“‚ Article πŸ“… 1982 πŸ› Elsevier Science 🌐 English βš– 785 KB

Variations of the ultrasensitive silver staining method of B. R. Oakley, D. R. Kirsch, and N. R. Morris (Anal. Biochem. 105, 361-363 (1980)) have been tested. It was established that the reliability of the method was greatly improved if (i) free silver was carefully washed out before reduction with

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✍ Dr. Carl R. Merril; David Goldman; Margaret L. Van Keuren πŸ“‚ Article πŸ“… 1982 πŸ› John Wiley and Sons 🌐 English βš– 763 KB

## Abstract The use of silver for detection of protein in polyacrylamide gels is becoming widespread. An effort has been made to develop a silver stain which minimizes the time to perform the stain, the amount of silver used, and the complexity of the procedures, and which maximizes the sensitivity