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Incorporation of methylamine in an ultrasensitive silver stain for detecting protein in thick polyacrylamide gels

✍ Scribed by Thomas Marshall; Albert L. Latner


Publisher
John Wiley and Sons
Year
1981
Tongue
English
Weight
848 KB
Volume
2
Category
Article
ISSN
0173-0835

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Staining of polyacrylamide gels with methylene blue prior to silver staining increases band resolution and sensitivity. This method permits resolution of multiple bands less than 1 mm apart, and is able to detect bands containing only 100 pg of RNA.