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An improved method for increasing the resolution and sensitivity of silver staining of nucleic acid bands in polyacrylamide gels

✍ Scribed by Gerald M. Kolodny

Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
495 KB
Volume
138
Category
Article
ISSN
0003-2697

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✦ Synopsis

Staining of polyacrylamide gels with methylene blue prior to silver staining increases band resolution and sensitivity. This method permits resolution of multiple bands less than 1 mm apart, and is able to detect bands containing only 100 pg of RNA.

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