The Staining of Acidic Proteins on Polyacrylamide Gels: Enhanced Sensitivity and Stability of “Stains-All” Staining in Combination with Silver Nitrate
✍ Scribed by Harvey A. Goldberg; Kevin J. Warner
- Publisher
- Elsevier Science
- Year
- 1997
- Tongue
- English
- Weight
- 482 KB
- Volume
- 251
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
Staining of polyacrylamide gels with methylene blue prior to silver staining increases band resolution and sensitivity. This method permits resolution of multiple bands less than 1 mm apart, and is able to detect bands containing only 100 pg of RNA.
A highly sensitive silver stain for visualizing proteins in polyacrylamide gels was adapted for staining 1,2-diol groups of carbohydrates. This periodic acid-silver stain is 64 times more sensitive than the periodic acid-Schiff stain, and the lower limit of detection is about 0.4 ng of bound carbohy