✦ LIBER ✦
A highly sensitive periodic acid-silver stain for 1,2-diol groups of glycoproteins and polysaccharides in polyacrylamide gels
✍ Scribed by Gérard Dubray; Guy Bezard
- Publisher
- Elsevier Science
- Year
- 1982
- Tongue
- English
- Weight
- 347 KB
- Volume
- 119
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
A highly sensitive silver stain for visualizing proteins in polyacrylamide gels was adapted for staining 1,2-diol groups of carbohydrates. This periodic acid-silver stain is 64 times more sensitive than the periodic acid-Schiff stain, and the lower limit of detection is about 0.4 ng of bound carbohydrates. Its lower limit of detection is comparable to autoradiography of radioactively labeled lipopolysaccharides.